张华;付强;钱亚云;刘延庆;李国清;崔平方;朱耀东;马慧;季雪;郭试瑜;久光正;

• 1:扬州大学中西医结合研究所
• 2:扬州大学医学院
• 3:河南中医学院
• 4:日本昭和大学医学部

摘要(Abstract)：

目的探讨南蛇藤提取物抑制人肝癌转移的分子机制。方法人肝癌HCCLM6细胞生长于培养液中进行传代,取对数生长期细胞进行实验。南蛇藤提取物组共分为10、20、40、80、160mg/L5个浓度,阳性对照组给予5-氟尿嘧啶(5-Fu)注射液180mg/L,阴性对照组不做处理。分别观察南蛇藤提取物对HCCLM6细胞增殖和迁移能力的影响,并检测南蛇藤提取物对HCCLM6细胞及其培养上清中血管内皮生长因子-C(VEGF-C)蛋白表达的影响。结果南蛇藤提取物组作用24、48h时160μg/ml对细胞增殖的抑制率最高(P<0.01)。南蛇藤提取物组和阳性对照组穿膜细胞数较阴性对照组明显减少(P<0.01);南蛇藤提取物组10、20、40、80μg/ml浓度穿膜细胞数多于阳性对照组,并呈浓度依赖性(P<0.05或P<0.01)。南蛇藤提取物组各浓度和阳性对照组VEGF-C蛋白水平均低于阴性对照组(P<0.05或P<0.01)。结论南蛇藤提取物能抑制HCCLM6细胞的增殖、迁移及下调VEGF-C蛋白的表达水平,这可能是其抑制肝癌转移的部分机制。

关键词(KeyWords)： 南蛇藤提取物;肝癌;增殖;迁移;血管内皮生长因子-C

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81173603,81274741);; 江苏省科技支撑计划(社会发展)资助项目(BE2011738);; 江苏省基础研究计划(自然科学基金)面上研究资助项目(BK2012686);; 江苏省中医药科技项目(LZ11210);; 扬州市产学研合作专项资助项目(YZ2010157)

作者(Author): 张华;付强;钱亚云;刘延庆;李国清;崔平方;朱耀东;马慧;季雪;郭试瑜;久光正;

Email:

DOI: 10.13288/j.11-2166/r.2013.10.026

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